Not before the petition is approved and a visa is available. The timeline runs from petition filing through adjudication, visa availability, consular processing and admission, then two years of conditional residence; the house is not needed to fund the capital and should not be sold to a project schedule.
Keep the family's irreversible decisions behind the government's decisions
The contractor's capital comes from the buyout and the land sale, so the family home is not part of the funding plan and does not need to be sold to file. That separation is worth protecting. The sequence on the immigration side is long: the investor petition is filed with the source-of-funds file, USCIS adjudicates it over a period that should be read from current processing information rather than a promoter's estimate, an immigrant visa must be available for Canadian-born applicants under the Visa Bulletin, the family completes consular processing, and only on admission does the two-year conditional period begin.
A regional center's construction schedule and its projected job creation are business milestones, not immigration dates. Selling the house, withdrawing the children from school or ending the wife's employment should each wait for the stage that requires them, generally after the visa interview. If the project's own timeline slips, the removal-of-conditions evidence may be affected, so ask the regional center in writing how it handles delays before committing.