Government filing charges at the investor-petition, immigrant-visa and removal-of-conditions stages, professional fees at each stage, business-plan and economic-support costs, document and translation costs, and the state dental-licensing process. Only the capital placed at risk in the clinic counts as the investment.
Where the standalone route spends differently
A standalone investor does not pay a regional center's administrative fee, but she pays for what the center would have supplied: a business plan that meets the standard reviewers apply, with a dated hiring schedule, patient-volume assumptions and financial projections, and often an accountant's or consultant's support for those assumptions. USCIS filing charges for the investor petition, adjustment of status if used, and the removal-of-conditions petition appear on Form G-1055; the Department of State publishes immigrant-visa fees for consular processing. Professional fees follow the same three stages, and a quote for the first stage alone is not the cost of the route.
The state dental-licensing process has its own fees and is not an immigration cost, but it belongs in the plan because the clinic's revenue depends on it. Keep the clinic's build-out and equipment in the investment column as capital at risk, and keep the family's relocation costs entirely outside it. Confirm government amounts at filing rather than at planning.