The regional center's administrative fee, the USCIS petition fees for the I-526E and later the I-829 as listed on the fee schedule, immigrant visa or adjustment fees per family member, legal fees, source-of-funds preparation with an accountant, translations and independent financial diligence. None of these count toward the investment.
List the fees that are certain and the ones that depend on the project
The published government charges include the I-526E petition fee, the later I-829 petition fee, and the applicable immigrant-visa or adjustment charges for family members; verify them on the current official schedules. Project-dependent charges include the sponsor's administrative fee and any subscription or escrow costs, whose amounts and refund terms must be read from the offering documents rather than supplied as a generic statistic.
Professional fees vary with the actual work, so ask whether a quote covers source-of-funds review and the I-829 stage. Add accounting work needed to reconstruct and reconcile the business history, plus independent financial review of the project, because immigration-file review is not an opinion that the investment will be repaid.